Yeast Display

We have adapted yeast display, mostly in the project on Armadillo Repeat Proteins. A new strategy in using yeast display has been devised, focusing mostly on specificity. It succeeds in competing off binders which predominantly recognize other targets (unspecific binders), but, even more importantly, also removes unselective binders, which, in addition to the correct target, also recognize related targets. Yeast display is using FACS, and the art of selection and counterselection has been pivotal in obtaining high-specificity binders with Armadillo Repeat Proteins.