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Vapor diffusion crystallization experiment
The Phoenix and Gryphon-LCP liquid handling system can routinely dispense a drop volume between 100nl and 250nl for both the protein sample and reservoir solution into the well of a MRC 3-lens crystallization plate. Up to three different protein samples can be dispensed on a single MRC 3-lens crystallization plate. The protein sample volume needed for the crystallization experiments depends on the drop volume and the number of plates:
Drop volume | Sample volume per plate | Sample volume for N plates |
100 nl | 9.6 ul | N*9.6 +20 ul |
150 nl | 14.4 ul | N*14.4 +20 ul |
... | ... | ... |
250 nl | 24.0 ul | N*24.0 +20 ul |
The sample volume required for 4 plates (96 Well), 3 drops of (100nl, 150nl, 200nl) is:
(4 x (0.1+0.15+0.2)uL x 96) +20 =192.8 uL
Leftover protein samples will be stored at 4°C for up to one month if indicated in the reservation calendar. Samples older than 1 month will be discarded without notice.
Microseeding crystallization experiment
Seedstock drop volume | Seedstock volume per plate | Seedstock volume for N plate |
20 nl | >2.5 ul | N*2.5 +20 ul |
50 nl | 5.0 ul | N*5.0 +20 ul |