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Department of Biochemistry

Sample Volume

Vapor diffusion crystallization experiment

The Phoenix and Gryphon-LCP liquid handling system can routinely dispense a drop volume between 100nl and 250nl for both the protein sample and reservoir solution into the well of a MRC 3-lens crystallization plate. Up to three different protein samples can be dispensed on a single MRC 3-lens crystallization plate. The protein sample volume needed for the crystallization experiments depends on the drop volume and the number of plates:

 

Drop volume Sample volume per plate Sample volume for N plates
100 nl 9.6 ul N*9.6 +20 ul
150 nl 14.4 ul N*14.4 +20 ul
... ... ...
250 nl 24.0 ul N*24.0 +20 ul

Example of sample volume calculation:

The sample volume required for 4 plates (96 Well), 3 drops of (100nl, 150nl, 200nl)  is:
(4 x (0.1+0.15+0.2)uL x 96) +20 =192.8 uL

 

Note:

Leftover protein samples will be stored at 4°C for up to one month if indicated in the reservation calendar. Samples older than 1 month will be discarded without notice.

Microseeding crystallization experiment

Seedstock drop volume Seedstock volume per plate Seedstock volume for N plate
20 nl >2.5 ul N*2.5 +20 ul
50 nl 5.0 ul N*5.0 +20 ul

Weiterführende Informationen

PCC-Pinboard

New employee at PCC

I am delighted to welcome Görkem Kurtuldu to PCC as a Senior Scientist. She will be stepping into the role as my designated successor. Görkem brings a fresh perspective to PCC, and I am excited to see the positive impact she will make.
Please join me in welcoming Görkem!

New screen:

What’s new:
PCC supports researchers with preparing custom screens through their pipetting service. The researcher can freely compose a screen with up to 30 different stock solutions.
Be sure to have a look and feel free to ask the PCC staff concerning this new service.

We had to adjust the prices for plates that are set up at 4°C due to the significantly higher wear and tear of the liquid handler stationed in the cold room.

The new screen Morpheus® Fusion (Product#: MD1-129) from Molecular Dimensions is now available at PCC as the initial screen GS087.

 

RockImager and Liquid Handler Status

RockImager 20°C:
The imaging schedule is on time.

RockImager 4°C:
The imaging schedule is on time.

Crystal Gryphon 4°C:
In Operation.

Crystal Gryphon LCP 20°C:
In Operation.

Crystal Phoenix 20°C:
In Operation.

Freedom EVO w/ MCA 96 + RoMa
In Operation

Freedom EVO w/ LiHa
In Operation

Contact

Department of Biochemistry
University of Zurich
Winterthurerstr. 190
CH-8057 Zurich
Office: Y44 J 30/34
xtal@bioc.uzh.ch


Görkem Kurtuldu
Tel.: +41 44 635 6557